Droplet Digital PCR is ideal for nucleic acid quantitation. The workflow is simple. The results are accurate and precise.

Combine your DNA samples and primers and probes with QuantaLife Master Mix to create 8 prepared samples. Load the 20 μL of your prepared samples into individual wells of the 8-channel disposable droplet generator cartridge (DG8™).

Load cartridge into the droplet generator to create an emulsion of ~20,000 monodisperse droplets for each of the 8 prepared samples.

Pipette emulsified samples from the DG8 to a conventional 96-well PCR plate and amplify to end point (40 cycles) using a standard thermal cycler.

Load plate into droplet reader and start your run. Each well is read serially. Droplets are sipped and streamed single-file into a capillary and past a two-color detector at the rate of at least 1,000 per second to determine which droplets contain a target (+) and which do not (–).

QuantaLife software reads the positive and negative droplets in each sample and plots the fluorescence droplet-by-droplet as shown below. The fraction of positive droplets determines the concentration of the target in the sample.

QuantaLife software allows you to visualize the data in a variety of ways and determine concentrations in copies/µL.